Melatonin, 5-methoxy-N-acetyltryptamine, is a hormone found in all living creatures from algae to humans, at levels that vary in a daily cycle. Many biological effects of melatonin are produced through activation of melatonin receptors,while others are due to its role as a pervasive and extremely powerful antioxidant with a particular role in the protection of nuclear and mitochondrial DNA.Melatonin is secreted by the pineal gland in the brain and is important in the regulation of many hormones in the body. Among its key roles, melatonin controls the body's circadian rhythm, an internal 24-hour time-keeping system that plays an important role in when fall asleep and when wake up. Darkness stimulates the release of melatonin and light suppresses its activity.
Fish Melatonin (MT) ELISA Kit employs a two-site sandwich ELISA to quantitate MT in samples. An antibody specific for MT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Fish Melatonin (MT) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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