The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 MAP kinase cascade.CAV1 and CAV2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. By using alternative initiation codons in the same reading frame, two isoforms (alpha and beta) are encoded by a single transcript from this gene.
Chicken Caveolin-1 (CAV1) ELISA Kit employs a two-site sandwich ELISA to quantitate CAV1 in samples. An antibody specific for CAV1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCAV1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CAV1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CAV1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Chicken Caveolin-1 (CAV1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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