DNA cross-link repair 1A protein (DCLRE1A) is involved in the repair of DNA interstrand cross-links. DNA cross-links suppress transcription, replication, and DNA segregation. DCLRE1A is a regulator of the mitotic cell cycle checkpoint. Alternative splicing results in multiple transcript variants.
Chicken DNA cross-link repair 1A protein (DCLRE1A) ELISA Kit employs a two-site sandwich ELISA to quantitate DCLRE1A in samples. An antibody specific for DCLRE1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDCLRE1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DCLRE1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DCLRE1A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Chicken DNA cross-link repair 1A protein (DCLRE1A) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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