N4BP1 (NEDD4 Binding Protein 1) is a Protein Coding gene. GO annotations related to N4BP1 include RNA binding. An important paralog of N4BP1 is KHNYN. Inhibitor of the E3 ubiquitin-protein ligase ITCH. N4BP1 acts by interacting with the second WW domain of ITCH, leading to compete with ITCHs substrates and impairing ubiquitination of substrates.
Chicken NEDD4-binding protein 1 (N4BP1) ELISA Kit employs a two-site sandwich ELISA to quantitate N4BP1 in samples. An antibody specific for N4BP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyN4BP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for N4BP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of N4BP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Chicken NEDD4-binding protein 1 (N4BP1) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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