Monoamine oxidase B, is a protein belongs to the flavin monoamine oxidase family. It is an enzyme located in the mitochondrial outer membrane. It catalyzes the oxidative deamination of biogenic and xenobiotic amines and plays an important role in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. This protein preferentially degrades benzylamine and phenylethylamine.Edmondson et al. described structural features of the human enzyme: it has a hydrophobic bipartite elongated cavity that (for the "open" conformation) occupies a combined volume close to 700 Å6. hMAO-A has a single cavity that exhibits a rounder shape and is larger in volume than the "substrate cavity" of hMAO-B.
Canine Monoamine oxidase B (MAOB) ELISA Kit employs a two-site sandwich ELISA to quantitate MAOB in samples. An antibody specific for MAOB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAOB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAOB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAOB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Canine Monoamine oxidase B (MAOB) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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