Cardiac troponin C (cTnC) is a 161-amino acid protein organized into two domains: the regulatory N-terminal domain (cNTnC, residues 1-86), the structural C-terminal domain (cCTnC, residues 93-161), and a flexible linker connecting the two domains (residues 87-92). Each domain contains two EF-hands, Ca2+-binding helix-loop-helix motifs exemplified by proteins like parvalbumin.[8] and calmodulin. In cCTnC the two EF-hand motifs constitute two high affinity Ca2+-binding sites. that are occupied at all physiologically relevant calcium concentrations. In contrast, only the second EF-hand in cNTnC binds Ca2+ with low affinity, while the first EF-hand Ca2+-binding site is defunct.
Canine Cardiac Troponin C (cTn-C) ELISA Kit employs a two-site sandwich ELISA to quantitate cTn-C in samples. An antibody specific for cTn-C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anycTn-C present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for cTn-C is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of cTn-C bound in the initial step. The color development is stopped and the intensity of the color is measured.
Canine Cardiac Troponin C (cTn-C) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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