Lipid phosphate phosphohydrolase 2 is a member of the phosphatidic acid phosphatase (PAP) family. PAPs convert phosphatidic acid to diacylglycerol, and function in de novo synthesis of glycerolipids as well as in receptor-activated signal transduction mediated by phospholipase D.This protein is similar to phosphatidic acid phosphatase type 2A (PPAP2A) and type 2B (PPAP2B). All three proteins contain 6 transmembrane regions, and a consensus N-glycosylation site.Phosphatidic Acid Phosphatase Type 2C has been shown to possess membrane associated PAP activity. Three alternatively spliced transcript variants encoding distinct isoforms have been reported.
Bovine Lipid phosphate phosphohydrolase 2 (PPAP2C) ELISA Kit employs a two-site sandwich ELISA to quantitate PPAP2C in samples. An antibody specific for PPAP2C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPPAP2C present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PPAP2C is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PPAP2C bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Lipid phosphate phosphohydrolase 2 (PPAP2C) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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