Osteocalcin (OC) is the most abundant noncollagenous protein in mature bone where it constitutes 1% to 2% of the total protein. Synthesized by osteoblasts, it is incorporated into the bone matrix.Osteocalcin is a 49-residue protein with three gamma-carboxyglutamic acid residues, at positions 17, 21 and 24. These three residues confer on it a very strong ability to bind to hydroxyapatite. Osteocalcin is a noncollagenous protein found in bone and dentin. It is secreted by osteoblasts and thought to play a role in mineralization and calcium ion homeostasis. It has been stipulated that osteocalcin may also function as a negative regulator of bone formation, although its exact role is unknown.
Bovine Undercarboxylated osteocalcin (ucOC) ELISA Kit employs a two-site sandwich ELISA to quantitate ucOC in samples. An antibody specific for ucOC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyucOC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ucOC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ucOC bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Undercarboxylated osteocalcin (ucOC) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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