Mannose-6-phosphate isomerase or phosphomannose isomerase is the enzyme that catalyses the interconversion of mannose-6-phosphate and fructose-6-phosphate. In eukaryotes MPI is involved in the synthesis of GDP-mannose, a constituent of N- and O-linked glycans and GPI anchors and in prokaryotes it participates in a variety of pathways, including capsular polysaccharide biosynthesis and D-mannose metabolism. MPII's belong to the cupin superfamily whose functions range from isomerase and epimerase activities involved in the modification of cell wall carbohydrates in bacteria and plants, to non-enzymatic storage proteins in plant seeds, and transcription factors linked to congenital baldness in mammals. Three classes of MPI have been defined
Bovine Mannose-6-phosphate isomerase (MPI) ELISA Kit employs a two-site sandwich ELISA to quantitate MPI in samples. An antibody specific for MPI has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMPI present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MPI is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MPI bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Mannose-6-phosphate isomerase (MPI) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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