Protein Mpv17 (MPV17) encodes a mitochondrial inner membrane protein that is implicated in the metabolism of reactive oxygen species. Mutations in MPV17 have been associated with the hepatocerebral form of mitochondrial DNA depletion syndrome (MDDS).
Bovine Protein Mpv17 (MPV17) ELISA Kit employs a two-site sandwich ELISA to quantitate MPV17 in samples. An antibody specific for MPV17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMPV17 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MPV17 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MPV17 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Protein Mpv17 (MPV17) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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