Tubulointerstitial nephritis antigen (TINAG) encodes a glycoprotein that is restricted within the kidney to the basement membranes underlying the epithelium of Bowman's capsule and proximal and distal tubules. Autoantibodies against this protein are found in sera of patients with tubulointerstital nephritis, membranous nephropathy and anti-glomerular basement membrane nephritis. Ontogeny studies suggest that the expression of Tubulointerstitial nephritis antigen is developmentally regulated in a precise spatial and temporal pattern throughout nephrogenesis.
Bovine Tubulointerstitial nephritis antigen (TINAG) ELISA Kit employs a two-site sandwich ELISA to quantitate TINAG in samples. An antibody specific for TINAG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTINAG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TINAG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TINAG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Bovine Tubulointerstitial nephritis antigen (TINAG) ELISA Kit listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
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