1, About Nano cHiPLC® Columns

Developed specifically for nanospray mass spectrometry (MS), our NanoLC systems provide speed, sensitivity, resolution and reliability for your research. （75 μm ID C18 Reverse Phase Nano Columns for the cHiPLC-Nanoflex System）

2, Features

1> For research use only. Not for use in diagnostic procedures.

SCIEX nano cHiPLC columns are made with robustness and ease-of-use in mind.  Our manufacturing process uses a unique weir structure to retain the stationary phase particles in the column. These weirs are more reproducible to fabricate, while their dead-volume is virtually zero (~13 pL). Compared with nanoLC columns constructed from fused silica tubing with end-frits made by sintering stationary phase particles, the cHiPLC columns are much more robust and far easier to handle and connect without introducing dead volumes.

1）Easy: Plug & play chip simplicity with the performance of a nano column

2）Extendable: Flexibility to switch between workflows and projects rapidly in multi-user labs

3）Every time: Reproducible results from day-to-day, column-to-column and lab-to-lab

2> Column design

Special care has been given to the design of both the trap-chips and analytical column-chips in order to achieve separations that are equal or better than separations obtained using packed capillaries. The use of fused silica allows for cylindrical channels for packing nanoLC columns and traps. Instead of conventional frits made out of fused stationary phase particles, our cHiPLC columns use a unique weir structure to retain the stationary phase particles in the column. These weirs are more reproducible to fabricate, while their dead-volume is virtually zero (~13 pL). In addition, adsorption of sample components that can occur with frit material is not an issue with these types of structures.

3> Patented connection system

Connections to and from each chip are made using a patented connection system that can connect up to seven channels to the outside world with a dead volume of less than 1 nl. The force used to connect the chip is pre-set, so that every time the user exchanges a chip, a specifically leak-free connection is obtained without any required user adjustments.

4> Increased column-to-column reproducibility

Besides the ease of replacing a nanoLC column or trap in seconds, the use of our cHiPLC columns also increases column-to-column reproducibility. All chips are exactly identical, and our packing procedure guarantees the best possible column-to-column reproducibility in nanoLC. This is of importance for applications where retention time stability over longer periods of time and over multiple columns is important. Examples are the use of retention time in combination with accurate mass in peptide/protein identification and scheduling MRM's for peptide quantitation in biomarker verification.

3, Accessories

Nano cHiPLC columns and trap columns are available in a range of stationary phases and column lengths.