Recombinant Trypsin (USP)
Catalog Number:RPT0202
CAS:9002-07-7
EC:3.4.21.4
Source:Porcine trypsin, expressed in E.coli
1.DESCRIPTION
Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. The optimum pH of trypsin is pH 7.0-9.0. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF , aprotinin, soybean trypsin inhibitor and by metal chelating agents, e.g. EDTA. It is typically isolated from the pancreas of animals. Animal-derived materials are now subject to more stringent regulations, as authorities strive to ensure safer biologics and pharmaceuticals, such as in insulin and vaccine production.
Recombinant porcine trypsin is a genetically engineered protein expressed in E.coli and purified by HPLC. It is manufactured under NSF ISO 9001-2008 and in accordance to good manufacturing practice (GMP) guidelines. No animal derived products are used in the fermentation, purification, and final formulation.
2.ADVANTAGES
（1）Animal origin free:No animal-derived material is used during production.
（2）High purity:
1) Recombinant porcine trypsin provides increased specific activity no less than 3800 USP units/mg pro.
2) Reduce production costs while improving manufacturing progresses.
3.MAIN FEATURES
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Source
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E. Coli
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Appearance
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Clear, Colorless to Yellowish solution
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Composition
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10mM HCl,20mM CaCl2, pH2.0-3.0
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Protein Concentration
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70 ±10 mg/ml
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Specific activity
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≥ 3800 USP units/mg pro.
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Purity（RP-HPLC)
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NLT 70% β-trysin, NMT 20% α-trypsin
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Bioburden
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≦100 CFU/ml
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UNIT DEFINITION：One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).
5.RECOMMEND USAGE
Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-9.0.
6.STORAGE INSTRUCTIONS
Recombinant trypsin solution should be stored under -70℃. It is stable within 24 months.
7.RELATED PRODUCTS
Recombinant trypsin (Lyophilized powder), Recombinant Human Trypsin, Sequencing Grade Modified Trypsin.
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