Introduction
Introduction:
Cannula implantation system is an accepted method of acute and chronic intracranial drug administration for animal experiment. Single cannula system includes guide cannula, dummy cannula, internal cannula and fixing screw. Together with RWD stereotaxic instruments, holders, fixing screws, PE cannulas, injection pumps and other related products, this system provide a set of simple and effective experimental tool of unilateral intracranial administration for neuroscientists. More importantly, RWD offer users can customized cannulas based on their specific experimental requirements.
Components:

Guide cannula
Guide cannula consists of a threaded cylindrical plastic pedestal and a stainless steel tube and it has to be pre-implanted into brain and fixed with dental cement. Both dummy cannula and internal cannula can be inserted into guide cannula individually.

Dummy cannula
Stainless steel wire and threaded plastic cap make a dummy cannula. It can be inserted into guide cannula and fixed by the screw to seal the guide cannula and prevent tissue fluid outflow after injection. The length of the stainless steel wire can be customized according to the actual needs.

Internal cannula
Internal cannula can be fixed into guide cannula through fixing screw and its tail end is connected with PE tube for injections. The length of internal

Fixing screw
Fixing screw fixes internal cannula to guide cannula.

Ordering Instructions:
Determine the target area for drug administration according to research requirements and identify the specific depth of the interested nuclei by referring to the rat or mouse brain atlas.
Choose a certain type of guide cannula according to the brain size and the solubility of the drug, and then confirm the length of the guide cannula according to the atlas of the brain nuclei. The length of the guide cannula refers to the length of the stainless steel tube below the plastic pedestal (marked as C in the diagram). This length of C can be customized. For more information, please refer to ordering information.
After confirming the external diameter and length of the guide cannula, the length of the dummy cannula should be defined (see “G2” in the diagram). Usually, after lock the dummy canula to the guide cannula by screw, the tip of the dummy cannula is flush or protruding 0.5mm. The length of protruding part (G2) can also be customized. (Special note: when 62002 is used with 62102, the tip of dummy cannula can flush or protrude, only when the length of the guide cannula is less than 15.20mm)
Determine the length of the internal cannula (see “G1”), usually, when the internal cannula is inserted into the guide cannula, the tip of the internal cannula is flush with or protruding 0.5mm that of the guide cannula. The length of protruding part (G1) can also be customized.
Ordering information (Unit: mm):
Cannula
Matching
|
Guide Cannula
|
Dummy Cannula
|
Internal Cannula
|
Fixing Screw
|
PE Tube
|
1
|
62001
|
62101
|
62201
|
62501
|
62301★
|
OD 0.64 x ID 0.45
|
OD 0.40
|
OD 0.41 x ID 0.25
|
OD 5.5 x L7.5
|
OD 0.97 x ID 0.58
|
2
|
62002
|
62102
|
62202
|
62502
|
62302▲
|
OD 0.56 x ID 0.38
|
OD 0.30
|
OD 0.36 x ID 0.20
|
OD 5.5 x L7.0
|
OD 0.85 x ID 0.42
|
3
|
62003
|
62102
|
62203
|
62502
|
62302▲
|
OD 0.48 x ID 0.34
|
OD 0.30
|
OD 0.30 x ID 0.14
|
OD 5.5 x L7.0
|
OD 0.85 x ID 0.42
|
4
|
62004
|
62104
|
62204
|
62502
|
62310
|
OD 0.41 x ID 0.25
|
OD 0.20
|
OD 0.21 x ID 0.11
|
OD 5.5 x L7.0
|
OD 0.61 x ID 0.28
|
Notes:★62301 PE tubes could be connected to syringes with needle size0.65-0.80mm in outer diameter.
▲62302 PE tubes could be connected to syringes with needle size0.45-0.65mm in outer diameter.
Screws
NO.
|
Cat. Number
|
Name
|
Remarks
|
1
|
62510
|
Screw-M1.0xL2.0mm
|
OD 1.0mm, 2.0mm in length
|
2
|
62511
|
Screw-M1.2xL2.0mm
|
OD 1.2mm, 2.0mm in length
|
3
|
62512
|
Screw-M1.4xL2.5mm
|
OD 1.4mm, 2.5mm in length
|
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