Test Principle
Canine Echinococcus Granulosus ELISA Kit is sandwich-antibody enzyme linked immunosorbent assay designed to detect the presence of antigen
to canine Echinococcus Granulosus in the canine stool. A microtitration format has been
configured by coating canine Echinococcus antibody in alternating columns on the plate.
Upon incubation of the test sample in the coated well, antigens specific to canine Echinococcus
Granulosus forms a complex with the coated viral antibodies. After washing away unbound
material from the wells, an anti porcine horseradish peroxidase conjugate is added
that binds to any canine antigens attached in the wells.
In the final step of the assay, unbound conjugate is washed away, and enzyme substrate and a
chromogen, TMB, are added to the wells. Stop the reaction by adding stop solution.
Within 15 minutes READ the absorbance at 450 nm using 630 nm as the reference wavelength
if available.
Enzyme immunoassays for the qualitative of antigens against canine Echinococcus Granulosus
the stool samples.
Materials Supplied
1 Microtiter Plate 1 pieces (96 wells)
2 Negative control 1 tube
3 Positive control 1 tube
4 Enzyme Conjugate 1 bottle
5 Washing buffer 2 bottle
6 Substrate A 1 bottle
7 Substrate B 1 bottle
8 Stop solution 1 bottle
9 Sample diluent 2 bottle
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