Malachite green ELISA kit is a competitive enzyme immunoassay for the quantitative
analysis of Malachite green in meat and water.The coupling antigens are coated on the micro-well stripes. The Malachite green in the sample and the coupling antigens coated on the micro-well stripes compete for the anti-Malachite green antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration.
The optical density (OD) value of the sample has a negative correlation with the Malachite green in it.
The measurement is made photometrically at 450 nm.
The absorption is inversely proportional to the Malachite green
concentration in the sample.
2. Technical specification:
Sensitivity: 0.05 ppb Detect time: Approx.75 min
Detect limit:
Tissue and water..........................................................................0.5 ppb
Cross-reaction rate:
Malachite green................................................................................100%
Leucomalachite Green .....................................................................95%
Crystal Violet .............................................................................. 100%
Leucocrystal Violet ......................................................................... 95%
Recovery rate:
Tissue................................................................................75 ± 10%
Water................................................................................80 ± 20%
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