1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Ractopamine in the pork , liver, urine.
The coupling antigen is pre-coated on the micro-well stripes.
The Ractopamine in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Ractopamine antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration.
The optical density (OD) value of the sample has a negative correlation with the Ractopamine in it.
This value is compared to the standard curve and the Ractopamine residues is subsequently obtained.
2. Technical specifications
Sensitivity: 0.1ppb
Detection limit
Tissue 0.5 ppb
Urine, serum 0.5 ppb
Feed 50 ppb
Recovery rate
Urine, serum 90±10%
Tissue 80±10%
Feed 80±15%
Cross-reaction rate
Ractopamine 100%
Terbutalin < 1%
Clenbuterol < 13%
Ractopamine < 1%
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