Estriol ELISA kit is a competitive enzyme immunoassay for the quantitative analysis of Estriol in feed, tissue and urine.
The coupling antigens are coated on the micro-well stripes. The Estriolin the sample and the
coupling antigens coated on the micro-well stripes compete for the anti-Estriol antibodies.
After the addition of the enzyme conjugate, the TMB substrate is added for coloration.
The optical density (OD) value of the sample has a negative correlation with the Estriol in it.
The measurement is made photometrically at 450 nm. The absorption is inversely proportional to
the Estriol concentration in the sample.
Technical specification:
Sensitivity: 0.05 ppb Detect time: Approx.45 min
Detect limit:
Shrimp,fish...........................................0.05 ppb
Pork,chicken,liver ...........................................0.5 ppb
Feed..........................................................1 ppb
Milk................................................................0.05 ppb
Cross-reaction rate:
Estriol...........................................................100%
Diethylstilbestrol........................... 90%
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