Brief
Pb ELISA test kit is an indirect competitive enzyme immunoassay for Screening and quantitative analysis of Pb water,food, and other smaples.
Pb was approved as a metal and the maximal residue level (MRL) of 10 ng/ml residues in water, MRLs of Pb are 100 µg/ml in hunman blood, respectively.
Principle
The assay is performed in plastic microwells, which have been coated with the coupling antigens. The standards or the sample solutions and the anti-Pb antibodies are added to the microwells. During the incubation, the free Pb molecules and the coupling antigens compete for the anti-Pb antibodies. Any unbound anti-Pb antibodies and the complex of Pb bound anti-Pb antibodies are then removed in a washing step. Then horseradish peroxidase labeled IgG are added to the microwells and bound by the bound anti-Pb antibodies. After an incubation time of 30 min, the non-bound horseradish peroxidase labeled IgG are removed in a washing step. The bound enzyme activity is determined by the addition of substrate/chromigen
(peroxide/tetramethylbenzidine). The enzyme converts the colorless chromogen into a blue product. The addition of the stop solution leads to a color change
from blue to yellow. The color intensity is measured photometrically at 450nm. The color development is inversely proportional to the Pb concentration in the sample.
Technical specification:
Sensitivity: 0.1 ppb Detect time: Approx.75 min
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