Recombinant Proteinase K
Catalog Number:RPK09
Cas:39450-01-6
EC:3.4.21.14
Alternate names:Protease K, Endopeptidase K, Tritirachium alkaline proteinas
DESCRIPTION
A serine protease that displays the ability to digest native proteins, thereby inactivating enzymes such as DNase and RNase without recourse to a denaturation process. It retains its activity in presence of SDS and urea. It is inactivated by diisopropyl fluorophosphates (DFP) and phenyl methane sulfonyl fluoride (PMSF).
ADVANTAGES
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Format
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Lyophilized
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M.W
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29.3kD
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Purity
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99%
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Activity
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30 units/mg
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DNAses and RNAses
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not detected
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UNIT DEFINITION
One unit is defined as the amount of enzyme that will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 mol tyrosine per min at 37°C, pH 7.5(color by Folin-Ciocalteu reagent).
RECOMMEND USAGE
Prepare recombinant Proteinase K with 50 mM Tris-HCl,2 mM Calcium acetate.The optimum pH is 8.0.
STORAGE INSTRUCTIONS
Store lyophilized at -20°C. Aliquot the product after reconstitution to avoid repeated freezing thawing cycles.
HANDLING
Do not take internally. Wear gloves and mask when handling the product! Avoid contact by all modes of exposure.
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