Recombinant Trypsin Solution

1.DESCRIPTION

Recombinant trypsin solution, is an animal-component free trypsin solution optimized for cell dissociation. It is formulated with recombinant human trypsin / porcine trypsin expressed in E.Coli and refolded and purified by chromatography. The sequence of recombinant human trypsin is same as human trypsin 2 sequence.

2.FEATURES AND BENEFITS

（1）Recombinant trypsin,Animal component-free, eliminate the risk of virus and mycoplasma contamination

Recombinant trypsin, no bacteria, mycoplasma, mold and virus was found and bioburden is conform to biological standard, eliminates the risk of viruses, or other potential adventitious agents.

（2）Recombinant trypsin

Recombinant trypsin owns a similar kinetic of cell detachment to native trypsin. Soybean trypsin inhibitors and other inhibitors work the same way with recombinant human trypsin as they do with native trypsin.

（3）High purity

Recombinant trypsin provides high specific activity and eliminates contaminated material found in 1:250 trypsin or other animal origin trypsin.

（4）High safety

The trypsin solution, ready-to-use, eliminates the risk of activity loss in the process of transport and storage. It also eliminates the contamination risk that a lyophilized powder has.

（5）Convenient

Recombinant trypsin solution is formulated at an optimal concentration to dissociate adherent cells.

（6）Good work without EDTA

No EDTA contained in buffer. And a good cell dissociation result can be obtained, and then eliminate EDTA influence on cell apoptosis experimental analysis.

3.METHOD OF USE

Step 1. Pour 1 ml buffer solution into EP tube containing trypsin powder.

Step 2. Dissolve the trypsin powder.

Step 3. Pour the solution into the vial containing buffer solution and mix. Best use it within 4 hours.

NOTES:

Note 1. Dissociate adherent cells under room temperature ; 37 ℃ is not recommended.

Note 2. If necessary, after dissolving, immediately dispense into suitable containers, then stored under -20℃. To use it, dissolve it at room temperature. 

Note 3. The content of trypsin is 2000 BAEE unit/ml as above prepared. If necessary, dilute it to 1000 unit/ml or others based on your experimental results.  

4.PRODUCTS INFORMATION

UNIT DEFINITION：One BAEE unit of trypsin activity will produce a Delta A253 of 0.001 per minute in a reaction volume of 3.0ml at pH7.6 and 25℃ (1cm light path). 1 USP unit = 3 BAEE unit.