Principle
Rabies Virus Ab ELISA Test Kit is an enzyme immunoassay designed to detect the presence of antibody to Rabies Virus in the Rabies serum. A microtitration format has been configured by coating Rabies Virus antigen in alternating columns on the plate. Upon incubation of the test sample in the coated well, antibody specific to Rabies Virus forms a complex with the coated viral antigens. After washing away unbound material from the wells, an anti-porcine: horseradish peroxidase conjugate is added that binds to any Rabies antibody attached in the wells. In the final step of the assay, unbound conjugate is washed away, and enzyme substrate and a chromogen, TMB are added to the wells. Stop the reaction by adding stop solution.
Within 15 minutes READ the absorbance at 450nm using 630nm as the reference wavelength if available.
Enzyme immunoassays for the qualitative of antibodies against Rabies Virus in dog or human serum.
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