1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Bambuterol in the pork , liver, urine. The coupling antigen is pre-coated on the micro-well stripes. The penbutolol in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Bambuterol antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Bambuterol in it. This value is compared to the standard curve and the Bambuterol residues is subsequently obtained.
2. Technical specifications
Sensitivity: 0.2ppb
Detection limit
Tissue.....................................0.5ppb
Urine, serum........................0.1 ppb
Feed.......................................1.0 ppb
Recovery rate
Urine, serum.......................90±10%
Tissue....................................80±10%
Feed......................................80±15%
Cross-reaction rate
Bambuterol............................ 100%
Terbutalin...........................<0.1%
Clenbuterol........................<0.1%
Dobutamine......................<0.1%
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