African swine fever (ASF) is an acute, highly contagious and virulent infectious disease caused by the African swine fever virus (ASFV). It has a high incidence rate and a mortality rate that can reach up to 100%. The World Organisation for Animal Health (OIE) has listed it as a notifiable animal disease, and China has classified it as a Category A animal disease.
The disease was first confirmed in Kenya, Africa, in 1921. Since 2007, African swine fever has occurred, spread, and become endemic in many countries worldwide, especially in Russia and its surrounding regions. In August 2018, China discovered its first case of African swine fever in Liaoning Province. Since then, the number of outbreaks has been increasing month by month. Within less than half a year, more than 130 outbreaks have occurred in most provinces across the country.
In addition to using specific primers, the real-time fluorescent quantitative reagent kit for African swine fever developed by GeneRadar also employs fluorescent probes. This ensures that fluorescence is generated when specific DNA sequences of the African swine fever virus are detected, facilitating interpretation. Since the primers and probes only target specific DNA sequences of the African swine fever virus, they do not react with the chromosomes of pigs or the nucleic acids of other pathogens.
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