Product Specification
For researchers and laboratory teams specializing in tumor immunology, infectious disease immunology, or autoimmune disorder studies, the Human Granzyme B One-Step ELISA Kit from Antbio Inc. is a high-reliability, time-efficient tool designed to accelerate the quantification of human Granzyme B. As a key serine protease primarily secreted by cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells, Granzyme B plays a pivotal role in mediating cell-mediated cytotoxicity—critical for eliminating tumor cells, virus-infected cells, and abnormal cells. Its expression levels serve as a core biomarker for evaluating immune effector cell activity, tumor immunotherapeutic efficacy, and immune-related tissue damage, making this kit indispensable for basic research and preclinical trials.
What differentiates this kit from traditional ELISA solutions is its innovative one-step protocol. By integrating the multi-step processes of conventional ELISA (including antigen-antibody incubation, washing, and signal development) into a single streamlined workflow, it significantly reduces assay time (cutting hands-on operation by up to 50% compared to standard kits) while minimizing human error caused by repeated sample handling. This ensures consistent, reproducible results that meet the strict standards of academic research and biopharmaceutical development.
Every component of the kit is manufactured under rigorous quality control measures. It uses high-specificity monoclonal antibodies against human Granzyme B and premium-grade reagents, guaranteeing exceptional sensitivity (with a detection limit as low as pg/mL levels) and minimal cross-reactivity with other proteases (such as Granzyme A or Perforin). The kit also maintains excellent batch-to-batch stability, a key factor for generating credible, publishable data. Additionally, it is fully optimized for accurate detection in a range of human biological samples, including serum, plasma, cell culture supernatants, and tissue homogenates—enhancing its versatility for diverse research scenarios.
Backed by Antbio’s long-standing expertise in developing application-focused immunoassay tools, the Human Granzyme B One-Step ELISA Kit is engineered to address the unique demands of immune effector function research. Whether you’re assessing the efficacy of immune checkpoint inhibitors, studying the mechanism of viral clearance by CTLs, or investigating abnormal cytotoxic activity in autoimmune diseases, this kit delivers the precision, efficiency, and ease of use needed to advance your research progress.
To access detailed product specifications (including detection range, sample volume requirements, and assay duration), pricing information, ordering guidelines, and dedicated technical support, visit the official product page via the link below:
Human Granzyme B One-Step ELISA Kit - Antbio
| Antigen |
Granzyme B |
| Immunogen |
Recombinant Protein |
| Antibody Type |
Recombinant mAb |
| Reactivity |
Hu |
| Purification |
Protein A |
| Stability & Storage |
2 to 8 °C as supplied.
|
Kit
| Precision |
Intra-assay: 7.0%；
Inter-assay: 9.1% |
| Sample type |
Cell culture supernatant |
| Assay type |
Sandwich (quantitative) |
| Sensitivity |
39.826 pg/ml |
| Range |
78.13 pg/mL – 5000 pg/mL |
| Recovery |
Cell culture supernatant: 112% |
| Assay time |
60 minutes |
| Species reactivity |
Human |
Background
Granzyme B (GrB) is a serine protease pivotal in cytotoxic immune responses, mediating apoptosis and immune surveillance. Structurally, GrB is synthesized as a 32 kDa inactive pro-enzyme (zymogen) and activated through proteolytic cleavage. Its active form features a conserved catalytic triad (His57, Asp102, Ser195) essential for enzymatic activity. Post-translational modifications, including N-linked glycosylation, enhance its stability and regulate intracellular trafficking. GrB functions by binding to mannose-6-phosphate receptors (MPRs) for endosomal uptake into target cells, where it synergizes with perforin to induce cell death. Advantages of this OneStep ELISA Kit Design: Simplified Workflow: Combines sample incubation and detection in a single step, eliminating intermediate washing and reducing hands-on time. High Efficiency: Achieves results within 60 minutes, ideal for high-throughput studies or urgent diagnostics. Enhanced Precision: Minimizes procedural variability and cross-contamination risks through optimized reagent formulation. Broad Compatibility: Validated for diverse biological matrices, ensuring flexibility in experimental design. This kit’s streamlined protocol and robust performance make it an indispensable tool for researchers and clinicians seeking reliable Granzyme B quantification in time-sensitive or resource-limited settings.
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