| Description | This kit adopts competitive ELISA method, which can specifically detect HIS tag protein, and is used for quantitative detection of recombinant protein with HIS tag. |
| Composition | | Item | Quantity | Storage Condition | | HRP conjugated anti-His tag Antibody 10X | 600 µL | 2-8ºC | | His tag protein Lyophilized Standards | 2 vials | 2-8ºC | | Assay Diluent #1 | 50 mL | 2-8ºC | | Wash Buffer 10X | 20 mL | 2-8ºC | | TMB Solution | 12 mL | 2-8ºC | | Stop Solution | 12 mL | 2-8ºC | | Pre-Coated 96-Well His tag competitor -Microplate | 12 x 8 well strips | 2-8ºC | | Plate Seal | 2 | 2-8ºC |
|
| Background | A polyhistidine-tag is an amino acid motif in proteins that typically consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein.The total number of histidine residues may vary in the tag from as low as two, to as high as 10 or more His residues. Histidine (His)-tag is widely used for affinity purification of recombinant proteins, but the yield and purity of expressed proteins are quite different. This kit is developed for rapid and high throughput detection of His-tagged proteins. |
| Storage Temp. | Store at 4°C for one year. Avoid repeated freeze/thaw cycles. |
中文
