| Usage | I. Materials to be Prepared: 1. 10% formalin fixative. 2. Distilled water. 3. Ethanol. II. Procedure (for reference only): 1. Conventional fixation, typically using 10% formalin, followed by conventional dehydration and embedding. 2. Dewax paraffin sections and place in distilled water; frozen sections are placed directly in distilled water. 3. Rinse in tap water for 2-3 minutes, then rinse twice in distilled water. 4. Place in an oxidizing agent at room temperature for 5-8 minutes, generally no longer than 10 minutes. 5. Rinse once in tap water, then rinse twice in distilled water. 6. Place the specimen in Schiff Reagent and stain in a dark place at room temperature for 10-20 minutes. 7. Rinse in tap water for 10 minutes. 8. Place the specimen in hematoxylin staining solution and stain the nuclei for 1-2 minutes. 9. Differentiate in acidic differentiation solution for 2-5 seconds. 10. Rinse with tap water for 10-15 minutes, then rinse with double-distilled water until the blue color returns. 11. Dehydrate with conventional ethanol in a stepwise fashion. Transparent with xylene, then mount with neutral gum.
III. Staining results:
| PAS-positive substances | Red or purple | | Cell nucleus | Blue | | Cytoplasm | Various shades of red |
Note: The color depth depends largely on the length of time the sample is exposed to the oxidant solution and Schiff Reagent.
Negative Control (Optional):
1. Dissolve 1g of amylase in 100mL of PBS (pH 5.3) and treat for 30-60 minutes. Then place the sample in the oxidant solution along with the other sections. The result should be negative.
2. (Alternative) Treat a saliva slide (filtered and used) for 30-60 minutes and place the sample in the oxidant solution along with the other sections. The result should be negative.
3. (Alternative) If the photograph is using its own sample, skip the oxidant solution step and place the sample directly in Schiff Reagent. The result should be negative. |
| Description | Glycogen staining is a common staining method in pathology. McManus first used the periodic acid-Schiff technique in 1946 to visualize mucin. This method is commonly used to visualize glycogen and other polysaccharides. This stain not only reveals glycogen, but also neutral mucinous substances and certain acidic substances, as well as cartilage, the pituitary gland, mold, fungi, pigments, amyloid, and basement membranes. Oxidant solutions can oxidize 1,2-ethylene glycol groups in carbohydrates and related substances, converting them to dialdehydes. The aldehydes then combine with Schiff's reagent to form a fuchsin compound, producing a purple-red color. Because oxidants can also oxidize other intracellular substances, careful selection of the oxidant concentration and oxidation time is crucial. This ensures that the oxidant groups are oxidized to aldehydes without overoxidation.
The characteristics of this glycogen PAS staining solution: it adopts our company's unique formula technology, which greatly enhances the staining effect; it has stable performance and strong specificity; it is simple to operate and only takes about 1 hour.
Product components:| Name | 4×50mL | 4×100mL | Save | | Reagent (A) Oxidizing agent | 50mL | 100mL | 4℃, protected from light | | Reagent (B) Schiff Reagent | 50mL | 100mL | 4℃, protected from light | | Reagent (C) hematoxylin staining solution | 50mL | 100mL | Room temperature, avoid light | | Reagent (D) Acidic Differentiation Solution | 50mL | 100mL | Room temperature |
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| General Notes | 1. Dewax sections as thoroughly as possible, otherwise the staining effect will be affected.
2. The oxidizing agent should not be used for too long; the optimal oxidation temperature is 18-22°C.
3. The oxidizing agent solution and Schiff Reagent should be stored airtight at 4°C, away from sunlight and air during use. Before use, it is best to remove the sections and return them to room temperature 30 minutes in advance, then use them in a dark place away from light.
4. The acidic differentiation solution should be replaced frequently. The differentiation time should be determined by the thickness of the sections, the type of tissue, and the freshness of the acidic ethanol differentiation solution. In addition, sufficient water rinsing time should be provided after differentiation.
5. The duration of exposure to the oxidizing agent solution and Schiff Reagent is crucial and should be determined by the thickness of the sections and the type of tissue.
6. This staining solution is commonly used for staining routine tissue sections. For staining cells or extremely thin sections, it is recommended to purchase the Glycogen PAS Staining Kit (for cells), as it contains lower concentrations of oxidizing agent and hematoxylin solution, which prevents overstaining. 7. Keep the staining time of frozen sections as short as possible. 8. For your safety and health, please wear a lab coat and disposable gloves when operating. |
| Storage Temp. | Store at 2-8℃, away from light. Valid for 6 months. |
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