| Description | This kit uses a competitive ELISA method. The microplate is coated with valproate conjugated antigen, and the sodium valproate standard or sample is added. Free sodium valproate and the sodium valproate conjugated antigen pre coated on the microplate compete with each other for the anti valproate antibody enzyme marker, which is developed with TMB substrate. The color changes from blue to yellow after adding the stop solution, The absorbance value is inversely proportional to the content of sodium valproate in the sample, and the content of sodium valproate in the sample is calculated by the standard curve.
Kit Composition:
1. Pre coated sodium valproate conjugated antigen detachable enzyme plate: 1 piece (12 well × 8 pieces)
2. Sodium valproate standard: 6 bottles (1ml/ bottle), the contents are: 0 ppb, 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb, 8.1 ppb
3. Anti sodium valproate antibody enzyme conjugate: 1 bottle (6ml)
4. Chromogenic solution a:1 bottle (6ml)
5. Chromogenic solution B: 1 bottle (6ml)
6. Termination solution: 1 bottle (6ml), 2m sulfuric acid
7. Sample diluent: 1 bottle (10×, 6ml) for sample dilution
8. Concentrated washing solution: 1 bottle (20×, 20ml) for plate washing
9. One copy of instructions. |
| General Notes | 1. Please read the instructions carefully before using the reagent kit
2. Do not use expired reagent kits
3. Before using the reagent kit, restore the reagent to room temperature (25± 2 ℃), and it is recommended to warm it up for at least 2 hours
4. The standard product contains sodium valproate, and special attention should be paid when using it. Gloves should be worn during operation
5. The termination solution contains sulfuric acid, which prevents skin burns and corrosion of clothing when used
6. The suction heads used for different standards and samples cannot be mixed, otherwise it will affect the test results
7. Reagents from different batch number kits should not be mixed; The suction heads used for different standards and samples should not be mixed, otherwise it will affect the experimental results
8. When diluting the sample, the sample diluent in this reagent kit must be used, otherwise it will affect the experimental results
9. Avoid foaming when mixing reagents
10. Samples tested positive in this kit should be confirmed using another method such as HPLC or GC/MS. |
| Storage Temp. | 1. The kit should be stored at 2-8 ℃ and should not be frozen.
2. Unused microplates should be sealed and stored dry. |
| Test Range | The standard curve ranged from 0.1ppb to 8.1ppb. |
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