Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti human ifn-γ Capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The ifn-&gamma in the sample; Bound to solid-phase antibody and detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, signal enhancer was added for incubation. After washing to remove unbound material, sa-hrp was added again. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response and ifn-&gamma in the sample; There is a positive correlation between the concentration of. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: Pre coated 96 well plates, standards, ifn-γ One copy of detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 0.04 pg/ml

Detection Range: 0.39-25 pg/ml

Recovery Range: 85-94%

Storage Method: 2-8 ℃

Standard Curve:

Background:

γ Interferon (IFN-γ)Is a soluble dimeric cytokine and is the only member of type II interferons. It is mainly secreted by natural killer (NK) and natural killer T (NKT) cells and plays a role in innate immunity; It is secreted by cd4th1 and CD8 cytotoxic T cells during antigen-specific immunity. IFN-γ Or type II interferons play an important role in innate and adaptive immunity against virus, some bacteria and protozoan infections. IFN-γ It is an important activator of macrophages and an inducer of type II major histocompatibility complex (MHCII) expression. IFN-γ The abnormal expression of Bcl-2 is associated with many autoinflammatory and autoimmune diseases. In addition to directly inhibiting viral replication, ifn-γ Its importance to the immune system is more reflected in its immune stimulation and immune regulation functions.