Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti-human IL-33 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL-33 present in the sample combines with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, signal enhancer was added for incubation. After washing to remove unbound material, sa-hrp was added again. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of IL-33 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: A copy of pre coated 96 well plate, standard, IL-33 detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 0.16pg/ml

Detection Range: 6.25-400 pg/ml

Recovery Range: 92-110%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Interleukin 33 (IL-33)Also known as nf-hev and DVS 27, it is a member of the IL-1 family with a molecular weight of 30 kDa and is secreted by many types of cells, including fibroblasts, mast cells, dendritic cells, macrophages, osteoblasts, endothelial cells, and epithelial cells. IL-33 can induce the secretion of type II cytokines by helper T cells, mast cells, eosinophils and basophils. IL-33 activates NF - & kappa by binding to receptor ST2 and IL-1 receptor accessory protein (IL1RAP); B and extracellular molecules in the MAP kinase signaling pathway, the latter can promote polarized Th2 cells to secrete type II cytokines (such as IL-5 and IL-13), thereby exerting their biological functions. After injection of IL-33, it can induce type II cytokines in vivo, which is thought to induce severe pathological changes in mucosal organs. The ligand / receptor complex appears to signal through the JAK STAT pathway.