Anti-Rabbit HRP-DAB IHC detection kit provides on step detection protocol, short incubation time and faster turnaround. Prior to staining the formalin-fixed paraffin tissue sections should be deparaffinized and hydrated following heat-induced epitope retrieval or enzyme pretreatment. Endogenous peroxidase should be blocked. Then add primary antibody and incubate at optimal titration and condition. After that add HRP Goat anti-Rabbit IgG (H+L) (Ready-to-use, specific for IHC) and incubate for 10-15 minutes. HRP will catalyze DAB to form visible brown deposit at the antigen site. When color development achieved satisfactory level, the slides are washed in water to stop reaction. The stained slides may be mounted with either aqueous mounting or organic mounting medium.
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