Human IL-27 ELISA Kit,Absin_specification/price/image

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Product Specification

Description

Detection Principle:

The double antibody sandwich ELISA method was used in this experiment. Anti human IL-27 monoclonal antibody is coated on the microplate. IL-27 in samples and standards will bind to the antibody fixed on the plate, and the free components will be washed away; Horseradish peroxidase labeled anti human IL-27 multi antibody was added to combine with IL-27 bound on the microplate to form an immune complex, and the free components were washed away; Add the substrate solution (chromogenic agent), the color of the solution gradually turns blue, add the stop solution, the solution turns yellow and stops changing. The absorbance was measured with a microplate reader.

Detection Type: Double antibody sandwich method

Form: pre coated 96 well plate

Detection Sample Type: cell supernatant, serum, Plasma

Loading Amount: 100ul

Kit Components:

Pre coated 96 well plate, standard, anti human IL-27 detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 1.8 pg/ml

Detection Range: 156 - 10000 pg/ml

Recovery Range: 82-110%

Storage Method: 2-8 ℃

Standard Curve

Background:

IL-27 is composed of ebi3 (associated with the P40 subunit of IL-12 and IL-23) and p28 (associated with the p35 chain of IL-12)Composed of heterodimeric cytokines. IL-27 is expressed by monocytes, endothelial cells, and dendritic cells through a receptor complex consisting of IL-27 R alpha/wsx-1/tccr and gp130. IL-27 induces IL-12 receptor expression on naive cd4+ T cells, sensitizing them to subsequent IL-12-induced Th1 development. It also acts as an anti-inflammatory cytokine by limiting the activity of T and NKT cells.

AntBio is a biotechnology group company dedicated to serving life sciences, aiming to help scientists accelerate research and improve work efficiency. AntBio provides comprehensive and high-quality reagent tools for basic research, drug development, and diagnosis, including research grade antibodies, proteins, biochemical reagents, and assay kits. These research tools are widely used in different segments of life science research. The group company currently consists of three brands, Absin, Starter-Bio and UA-Bio.