| Description: |
M-MuLV Reverse Transcriptase is a modified and optimized Moloney Murine Leukemia Virus (M-MuLV) Reverse Transcriptase. It is a DNA polymerase that uses RNA or DNA as template to synthesize complementary DNA strands in the presence of primers. It also has Ribonuclease H (RNase H) activity, which can specifically degrade the RNA in RNA-DNA hybrids, but not single-stranded RNA or double-stranded RNA. |
| Origin： |
E. coli. |
| Activity： |
One unit of the enzyme incorporates 1 nmol of dTMP into a polynucleotide fraction in 10 min at 37℃. Enzyme activity is assayed in 50 mM Tris-HCl (pH 8.3), 6 mM MgCl2, 10 mM DTT, 40 mM KCl, 0.5 mM dTTP, 0.4 MBq/ml [3H]-dTTP, 0.4 mM polyA•oligo(dT)12-18 |
| Purity: |
E. coli. |
| Formulation: |
Supplied as 50 mM Tris, pH 8.3, 100mM NaCl, 1 mM EDTA, 5 mM DTT, 0.1% Triton X-100 and 50% glycerol. |
| Storage: |
Store at -20℃. Avoid repeated freeze/thaw cycles |
| Reaction： |
250 mM Tris, pH 8.3 at 25℃, 250 mM KCl, 20 mM MgCl2, 50 mM DTT |
| Applications: |
First strand cDNA synthesis using total RNA or mRNA as template; DNA probe labeling; RNA analysis by primer extension. |
| Packing： |
5000U |
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