Human Papillomavirus Genotyping qPCR Kit,ECALBIO_specification/price/image

Product Detail
Company Profile

1  Principle And Intended Use
This product is used for the typing and qualitative detection of 21 subtypes of human papillomavirus (specifically including HPV 16, 18, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 26, 82, 73, as well as HPV 6, 11, and 81) in cervical exfoliated cell samples from women.

2 Specifications
   12 tests/plate， 5 plates/kit ( 60 tests)

3 Components
  Each kit contains 1 pre-packaged reagent 96-well plate, 1 microplate sealers,
  1 HPV Positive Control, 1 HPV Negative Control, and 1 instruction.

4 Storage Condition and Expiring Date
The reagents are stable when stored at room temperature for 12 months without unpacking. Please transport at
  -25℃ to 35℃. The kit should remain sealed and away from light exposure. Do not use the kit beyond the
   expiration date.

5 PCR Assay
①　Create a new experiment.
②　Select the FAM and VIC channels.
③　Select “none” for Passive Reference (For ABI Real-Time PCR systems).
④　Set cycle conditions. Enter the reaction volume (25μL), and edit the PCR program as follows:
Step 1：50℃ 2min；
Step 2：95℃ 5min；
Step 3：95℃ 10s，58℃ 40s (fluorescence collection ).（40 cycles）
Save the file. Run the program.

⑤　Save the file. Run the program.
   Each well of the reagent strip detects different targets respectively:

Well	Sigal	HPV genotype
A	FAM	HPV16
	VIC	HPV18
	CY5	HPV31
B	FAM	HPV59
	VIC	HPV66
	CY5	HPV53
C	FAM	HPV33
	VIC	HPV58
	CY5	HPV45
D	FAM	HPV56
	VIC	HPV52
	CY5	HPV35
E	FAM	HPV68
	VIC	HPV51
	CY5	HPV39
F	FAM	HPV82
	VIC	HPV26
	CY5	HPV73
G	FAM	HPV6
	VIC	HPV11
	CY5	HPV81
H	VIC	Internal reference