Accurate and efficient: multi-color LED light source excitation, PD detector to detect fluorescence, the result is accurate and true.
Multi-color and multiple: four-color fluorescence, supports FAM/SYBR Green, VIC/HEX/JOE, ROX/Texas Red, Cy5 and other dyes; supports up to 28 targets/genes in a single PCR tube.
A POCT platform: support for sample testing and report upon arrival without waiting. A single instrument comes with four independent units, which can detect four samples or four items simultaneously or independently, and one detection unit does not interfere with the others. Each unit both has a faster run speed and a shorter turnaround time.
Complete functions: support qualitative detection, quantitative detection, genotyping, melting curve, etc.
Touch screen APP: simple and easy to use, easy to get started.
Mobile and portable: compact design, light weight, convenient to move and carry, can work in a car, suitable for different application scenarios.
High safety: It can be equipped with a automatic nucleic acid extractor to extract nucleic acid (DNA/RNA), and the screw cap tube-type PCR chip can be fully enclosed before detection to prevent pathogens and aerosol leakage, which can effectively ensure the safety of personnel and the environment.
t400 supporting microfluidic chip: Totalchip™ PCR Chip
Single tube multiple: achieve real-time PCR reaction with no less than 20 targets/genes (20 multiples) in a single PCR tube.
Ultra-numerous targets/genes: based on TaqMan® probes, 20 targets/genes can be detected simultaneously.
Extended targets/genes: what is more, 80-120 targets/genes can be detected based on melting curve method.
High sensitivity: although the PCR reaction is still carried out in a single tube, the detection sensitivity of 20 targets/genes is uncompromising compare to that of a single targets/genes.
| Model |
t400 |
| Physical |
| Dimension (W × D × H) |
24.0 cm × 34.4 cm × 18.4 cm (9.4" × 13.5" × 7.2") |
| Net weight |
5.0 kg(11.0 lbs) |
Power supply,
Consumption |
12V 11.5A |
| Communication |
USB 2.0 ×2 |
| Display |
8" color touchscreen |
| Language |
Chinese |
| Environmental |
| Operating conditions |
<Temperature: 10~30℃ (50~86℉);
Relative Humidity: 20%~80% non-condensing |
| Storage & transport conditions |
ATemperature: -20~55℃ (-4~131℉);
Relative Humidity: 20%~80% non-condensing |
| Altitude |
<2,500 m (8202 feet) |
| Reaction Vessels |
| Sample capacity |
4×60μL (Single tube per sample block) |
| PCR tube type |
TotalchipTM PCR Chip |
| Reaction volume |
60μL |
| Thermal Performance |
| Heating/cooling method |
Aluminum-based hot plate heating/fan cooling |
| Temperature range |
RT~99℃ |
| Peak heating rate |
>12℃/sec |
| Average heating rate |
>10℃/sec |
| Peak cooling rate |
>3.0℃/sec |
| Average cooling rate |
>2.0℃/sec |
| Temp precision |
<±0.15℃ |
| Temp accuracy |
<±0.15℃ |
| Optical Performance |
Number of
detection channel |
4 |
| Excitation source |
High brightness, 4 colored LEDs |
| Detector |
High sensitive, high signal-to-noise ratio photodiodes |
| Dyes detected |
F1: FAM, SYBR Green, Fluo-4, FITC, LC Green, Alexa Fluor 488, Oregon Green 488, NBD, EGFP;
F2: HEX, JOE, VIC , Alexa Fluor 532;
F3: ROX, Cy3.5, Texas Red, Alexa Fluor 594;
F4: Cy5, Atto 633, Alexa Fluor 633, LC Red 640 |
| Parameter |
| Detection sensitivity |
Down to one copy |
| Dynamic range |
101~109 (assay dependent) |
| Linearity |
≥0.99 |
| Channel crosstalk |
No crosstalk |
| Test repeatability |
≤1.5% |
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