Test principle
The basis of the test is on the competitive enzyme immunoassay for the detection of Diclofenac in the serum and perilymph sample. The microtiter wells are coated with anti-Diclofenac antibody. Diclofenac enzyme conjugate and the Diclofenac standards or sample solution are added. Free Diclofenac antibody and Diclofenac enzyme conjugate compete for the Diclofenac antibody binding sites (competitive enzyme immunoassay). Any unbound enzyme conjugate is then removed in a washing step. Substrate solutions are added to the wells, bound enzyme conjugate converts the colorless chromogen into a blue product. The addition of the stop reagent leads to a color change from blue to yellow. The depth of the color shows the magnitude of Diclofenac in the sample.The measurement is made photometrically at 450nm. According to the absorbance, the concentration of Diclofenac in the sample can be quantified.
Technical specification:
Sensitivity: 3.0ppb
Detect time: Approx.45 min
Detect limit:
Tissue.....................................................40ppb
Serum,Perilymph ...............................80ppb
Recovery rate
Tissue......................................................85 ± 15%
Serum,Perilymph.................................85 ± 20%
ECALBIO CO.,LTD
Email: info@ecalbio.com Https: //www.ecalbio.com
Tel: +86-27-87745856 Mobi: +86-18963964485
Add: HZAU, Luoshi Road, Wuhan City 430075, China.
bio-equip.cn
中文
