1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of Vancomycin in the tissue, liver, honey, intestine, shrimp, fish, etc. The coupling antigens are pre-coated on the micro-well stripes. The Vancomycin in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Gen antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with Vancomycin concentration in the sample. This value is compared to the standard curve and the content of the corresponding Vancomycin is subsequently obtained.
2. Technical specifications
Sensitivity : 1ppb Detect time: Approx. 75min
Detection limit:
Tissue, liver, intestine .........................3ppb
Milk ...........................................20ppb
Recovery rate:
Tissue, liver, intestine,milk ....... 85±10%
Cross-reaction rate:
Vancomycin.......................100 %
ECALBIO CO.,LTD
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