Description
Immortalized Human Hepatic Stellate Cells-SV40 were developed from human tissues transduced with a lentiviral expression vector containing the SV40T gene. The cell line was continuously cultured for more than 30 passages without showing signs of growth retardation or replicative senescence.
Recommended Medium
SuperCult® Immortalized Human Hepatic Stellate Cell Medium (Cat No.: CM-I2096Z)
Freezing Medium
Complete medium supplemented with 10% (v/v) DMSO
Culture Properties
Adherent
Immortalization Method
SV40 large T antigen and Human telomerase reverse transcriptase (hTERT)
Growth Properties
Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2.
Shipping
Dry Ice.
https://www.creative-bioarray.com/immortalized-human-hepatic-stellate-cells-sv40t-csc-i2096z-item-46028.htm