Human Obestatin ELISA Kit
Cat NO. PRS-01916hu
FOR RESEARCH USE ONLY. Not FOR CLINICAL DIAGNOSIS USE.
Purpose
This kit allows for the determination of Obestatin concentrations in Human serum, blood plasma, and other biological fluids.
Principle of the assay
The kit assay Human Obestatin level in the sample，use Purified Human Obestatin antibody to coat microtiter plate wells, make solid-phase antibody, then add Obestatin to wells, Combined Obestatin antibody which With HRP labeled，become antibody-antigen- enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Obestatin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Materials provided with the kit
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Materials provided with the kit
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48determinations
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96 determinations
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Storage
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User manual
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1
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1
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Closure plate membrane
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2
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2
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Sealed bags
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1
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1
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Microelisa stripplate
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1
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1
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2-8℃
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Standard：360 pg/ml
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0.5ml×1 bottle
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0.5ml×1 bottle
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2-8℃
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Standard diluent
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1.5ml×1 bottle
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1.5ml×1 bottle
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2-8℃
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HRP-Conjugate reagent
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3ml×1 bottle
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6ml×1 bottle
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2-8℃
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Sample diluent
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3ml×1 bottle
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6ml×1 bottle
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2-8℃
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Chromogen Solution A
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3ml×1 bottle
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6ml×1 bottle
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2-8℃
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Chromogen Solution B
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3ml×1 bottle
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6ml×1 bottle
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2-8℃
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Stop Solution
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3ml×1 bottle
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6ml×1 bottle
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2-8℃
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wash solution
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（20ml×20 fold）
×1bottle
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（20ml×30 fold）
×1bottle
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2-8℃
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