Purpose
This kit allows for the determination of F1+2 concentrations in Human serum, blood plasma, and other biological fluids.
Principle of the assay
The kit assay Human F1+2 level in the sample，use Purified Human F1+2 antibody to coat microtiter plate wells, make solid-phase antibody, then add F1+2 to wells, Combined F1+2 antibody which With HRP labeled，become antibody-antigen- enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of F1+2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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