Human Glucagon,GC ELISA Kit
Cat NO. PRS-00665hu
FOR RESEARCH USE ONLY. Not FOR CLINICAL DIAGNOSIS USE.
Purpose
This kit allows for the determination of GC concentrations in Human serum, blood plasma, and other biological fluids.
Principle of the assay
The kit assay Human GC level in the sample，use Purified Human GC antibody to coat microtiter plate wells, make solid-phase antibody, then add GC to wells, Combined GC antibody which With HRP labeled，become antibody-antigen- enzyme-antibody complex, after washing Completely, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of GC in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Materials provided with the kit
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Materials provided with the kit
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48determinations
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96 determinations
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Storage
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User manual
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1
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1
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Closure plate membrane
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2
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2
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Sealed bags
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1
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1
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Microelisa stripplate
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1
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1
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2-8℃
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Standard：225pg/ml
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0.5ml×1 bottle
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0.5ml×1 bottle
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2-8℃
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Standard diluent
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1.5ml×1 bottle
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1.5ml×1 bottle
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2-8℃
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HRP-Conjugate reagent
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3ml×1 bottle
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6ml×1 bottle
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2-8℃
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Sample diluent
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3ml×1 bottle
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6ml×1 bottle
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2-8℃
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Chromogen Solution A
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3ml×1 bottle
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6ml×1 bottle
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2-8℃
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Chromogen Solution B
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3ml×1 bottle
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6ml×1 bottle
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2-8℃
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Stop Solution
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3ml×1 bottle
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6ml×1 bottle
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2-8℃
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wash solution
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（20ml×20 fold）
×1bottle
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（20ml×30 fold）
×1bottle
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2-8℃
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Assay range
7 pg/ml -200 pg/ml
Storage and validity
1．Storage： 2-8℃.
2．validity： 12 months.
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