Ciprofloxacin (CPFX) ELISA Test Kit
【Specification】
96T/kit 96T*5/kit
【Principle of Test】
The Ciprofloxacin (CPFX) ELISA Test Kit is based on a competitive colorimetric ELISA assay.
The ciprofloxacin antibody has been coated in the plate wells. During the analysis, sample is added along with the ciprofloxacin-horseradish peroxidase (Ciprofloxacin-HRP) conjugate. If the ciprofloxacin residue is present in the sample, it will compete for the ciprofloxacin antibody, thereby preventing the Ciprofloxacin-HRP from binding to the antibody attached to the well, last, take coloration with TMB substrate. The samples A value is an inverse relationship with CPFX residue content. Lastly, compared with the standard curve can be concluded the CPFX residues in sample.
【Composition of the Kit】
Microelisa Stripplate, Standard , Enzyme conjugate, Antibody working solution , Concentrated Wash Solution 20×, concentrated Redissolving solution , Substrate A solution, Substrate B solution, Stop Solution.
【Technique Data】
1. Kit sensitivity: 0.1 ppb (ng/ml)
2.Reactive mode: 25℃, 45min～15min
3.Detection Limits:
|
Sample
|
Detection Limits
|
|
Tissue(chicken, pork, fish, shrimp)
|
0.3ppb
|
|
Honey
|
0.4ppb
|
|
Milk
|
3ppb
|
|
Milk powder
|
6ppb
|
|
Egg
|
3ppb
|
4.Cross-reaction rate:
|
Name
|
Cross-reaction rate
|
|
Ciprofloxacin
|
100%
|
|
Oxolin acid
|
28%
|
|
Levofloxacin
|
10%
|
|
Lomefloxacin
|
4%
|
|
Marbofloxacin
|
4%
|
|
Sarafloxacin
|
110%
|
5. Recovery rate:
Tissue, honey, eggs, milk, milk powder ……………85±15%
【Storage Conditions】
1.The kit shall be stored at [2-8 ℃]. Expiry date: 12 months
2.The opened Microelisa Stripplate can be stored at [2-8 ℃] and avoid damp. Use for at least 2 months.
months.
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