Creative Biolabs provides various techniques such as flow cytometry-based FRET, immunoprecipitation and western blotting for TCR pairing assay. Mispaired TCR heterodimers may result in the diluted expression of TCR transgene or the generation of autoreactive TCR, which affect the efficacy and safety of TCR engineered T cell therapy subsequently. It is a challenge to optimize TCR pairing issue. Creative Biolabs' various methods of to assess TCR pairing and evaluate efficacy and safety of TCR transfer.
https://www.creative-biolabs.com/car-t/tcr-pairing.htm