The most Advanced Flow FFF Platform for Separation of Proteins, Macromolecules and Nanoparticles!

Professional Modular Platform

The new Postnova AF2000 MultiFlow FFF Series was invented to become the first professional modular Flow FFF platform available. It is completely integrated by the NovaFFF single software platform which runs the entire system from autosampler to detectors. The AF2000 Series incorporates the combined solid know-how and the technologies from three decades of leadership in FFF. Due to its unique design, the AF2000 MultiFlow platform offers more flexibility, higher robustness and better performance than any system before. The technology sets a new standard in the area of Flow FFF and marks a real break-through in FFF science and therefore naturally has become the leading Flow FFF solution for many scientists around the world.

Universal Flexible Separator

The AF2000 Series platform is based on the Flow FFF principle, using a crossflow field as driving force for the separation. The samples, which are affected by this field, are separated by their Dynamic Diffusion on the basis of the molar mass or particle size. The AF2000 can be run with different eluents, at various temperature conditions and is able to employ various channel dimensions and principles. The same system can be used for running planar Asymmetric Flow FFF (AF4) channels or circular HollowFiber Flow FFF (HF5) channels. Therefore, the AF2000 MultiFlow can be easily used for separation of a broad range of different samples from the area of Biopharmaceuticals, Food-Agro-Cosmetics, Environmental, Chemicals and Nanotechnology. The MultiFlow FFF is ideal for the characterization of proteins, antibodies, aggregates, vaccines, liposomes, nanoparticles and natural or synthetic macromolecules. Because of its great flexibility, the AF2000 MultiFlow FFF can be employed as a Universal Separator for many kind of analyte systems and is a secure investment also for new upcoming samples and future project demands.

AF2000 AT - Standard Ambient Temp Flow FFF

The AF2000 AT is a non-temperature controlled economical Flow FFF which is ideal for the use in general level research in universities, companies and public scientific centers. It is available as aqueous metal-free, stainless steel and multisolvent version and can be run with a broad range of aqueous and organic solvents. Planar Asymmetric Flow FFF (AF4) and circular HollowFiber Flow FFF (HF5) channels can used at the same time. Various detection principles such as UV-Absorbance (UV), Refractive Index (RI), Multi-Angle Light Scattering (MALS), Dynamic Light Scattering (DLS) and Inductively Coupled Plasma Mass Spectroscopy (ICP-MS) can be coupled easily.

AF2000 MT - Advanced Mid Temp Flow FFF

The AF2000 MT is a temperature controlled advanced Flow FFF system which is ideal for the use in higher level research in university and public research institutions as well as professional research and commercial-industrial applications in the area of product development, manufacturing and quality control. It is also available as aqueous metal-free, stainless steel and multisolvent version and can be run with a broad range of aqueous and organic solvents. Planar Asymmetric Flow FFF (AF4) and circular HollowFiber Flow FFF (HF5) channels can used at the same time. Various detection principles such as UV-Absorbance (UV), Refractive Index (RI), Multi-Angle Light Scattering (MALS), Dynamic Light Scattering (DLS) and Inductively Coupled Plasma Mass Spectroscopy (ICP-MS) can be coupled easily. Temperature control is not only available for the separation channel but also for the entire system, including the transfer capillaries between the various modules and detectors.

AF2000 HT - Top-Level High Temp Flow FFF

The AF2000 HT is a special temperature controlled top-level Flow FFF system which is ideal for the use in research, development and quality control of polyolefins and high temperature macromolecules in universities, public research institutions and industry. It is available as stainless steel version and can be run with organic solvents from ambient temperature up to high temperature conditions. Special planar Asymmetric Flow FFF (AF4) channels made from stainless steel and ceramics are exclusively employed for this conditions. Infrared (IR), Refractive Index (RI), Multi-Angle Light Scattering (MALS) and Viscometry (Visco) can be used as detection principles .

Advanced Flow FFF Detection

In order to enable the user to fully access this wide application range, the AF2000 MultiFlow is available in different unique versions. Various add-on modules, extra options and special FFF detectors are available, such as Refractive Index (RI), Multi-Angle Light Scattering (MALS) and Dynamic Light Scattering (DLS). All Postnova FFF modules and detectors have been especially developed for FFF and are perfectly optimized not only for the use with the AF2000 MultiFlow FFF but also for the other Postnova FFF versions. Additionally, the AF2000 Series has been designed for easy interfacing with existing high-end detection technologies from leading instrument suppliers so that the user can ideally integrate the Postnova FFF technology into the existing laboratory infrastructure in order to maximize efficiency and investment.

Application Range

The AF2000 AT is the basic modular system which marks the Entry Level of the Postnova AF2000 MultiFlow platform. The system is non-temperature controlled and runs at typical ambient laboratory conditions from 15° to 25°C. It is ideal for standard research applications in university research groups and educational institutions with a clear focus and defined application range. The AF2000 is the best choice and a good starting point for groups which want to enter into the area of Field-Flow Fractionation for the first time. It is available as aqueous metal-free, stainless steel and multisolvent version and can be run with a broad range of aqueous and organic solvents. Planar Asymmetric Flow FFF (AF4) and circular HollowFiber Flow FFF (HF5) channels can used at the same time. Various detection principles, such as UV-Absorbance (UV), Refractive Index (RI), Multi-Angle Light Scattering (MALS), Dynamic Light Scattering (DLS) and Inductively Coupled Plasma Mass Spectroscopy (ICP-MS) can be coupled easily online.

System Hardware

It is the Postnova's clear mission to only offer fully optimized and professional FFF hardware, software and support solutions. Therefore, the AF2000 AT series is a basic but completely optimized Flow FFF system which contains all necessary standard modules and components in the package that are required for a robust, reliable and fully operable Flow FFF system. There is no need for assembling and utilizing semi-suited third party HPLC components which are often just made for other purposes, show less flexibility and are not available in the strict specifications needed for FFF applications. The AF2000 AT series is a true ready-to-use non-compromise Flow FFF system where no additional components are required.

The system incorporates the complete set of Postnova's proven FFF technologies which makes it the most easy-to-use, robust and reproducible system available. The AF2000 AT system is comprising an Eluent Organizer (PN7205), an Eluent Degasser (PN7520), an Eluent Pump (PN1130), a Focus Pump (PN1130), a Flow FFF Control Module with integrated electronic interface and cross-flow syringe pump (AF2000 Module), a Manual Injector Holder (PN5120), a user-selectable Asymmetric / HollowFiber Flow FFF channel (AF4/HF5) and a Control PC with the NovaFFF software suite for operation of the AF2000 AT system.

Additional System Options

The AF2000 AT Flow FFF is available in different versions and additionally can be upgraded and customized for special applications by adding extra system options. This can be done at the date of purchase or later as an upgrade.

EFS Eluent Filtration System

UVD Eluent Disinfection Module

SAM Manual/Auto Injector with Options

ELC Aqu. Metal-free/Organic/MultiSolvent

AF4/HF5 Channel Versions and Membranes

S3 Outlet Splitting Sensitivity Enhancer

RID Refractive Index Detection System

UVD Absorbance Detection System

FLD Fluorescence Detection System

MALS Multi-Angle Light Scattering Detector

DLS Zetasizer* Dynamic Light Scattering Detector

ICP-MS Online Connection and Interface

FRA Automatic Fraction Collector with Options

System Software

The unique NovaFFF single software platform is used for full control and data acquisition of the entire AF2000 AT Flow FFF together with associated peripheric modules (such as Auto Injector, Fraction Collectors) and detectors (LS, RI, UV, FLD). The software also allows comprehensive data analysis and evaluation based on Relative Standard Calibration and Absolute Light Scattering methods. Furthermore, the software is also the platform to run all major FFF versions, including AF2000 MultiFlow FFF, CF2000 Centrifugal FFF and TF2000 Thermal FFF. This, together with additional features, makes NovaFFF the most advanced and leading software platform available for FFF applications today.

Service & Support

In order to assist our users in achieving the best results and getting the maximum output from their FFF system without any time delay, Postnova offers a series of differently adjusted services and support activities. These services have been optimized to perfectly fit each step of the live cycle of any FFF system, from installation and initial training, via maintenance during operation all the way to relocation and upgrade services to keep up with new technological developments.

Installation Services

User Training Courses

System Maintenance

Relocation Services

Upgrade & Update Services