Furazolidone (AOZ) ELISA Test Kit
【Specification】
96T/kit 96T*5/kit
【Principle of Test】
The Furazolidone (AOZ) ELISA Test Kit is using indirect competitive enzyme immunoassay method.
The Furazolidone coupling antigen has been coated in the plate wells. During the detection, add Standards or samples to the well, AOZ of samples will compete with coated AOZ coupling antigen to combine with antibody, after adding antibody working solution, Horseradish Peroxidase (HRP), take coloration with TMB substrate. The samples A value is an inverse relationship with AOZ residue content. Lastly, compared with the standard curve can be concluded the AOZ residues in sample.
【Composition of the Kit】
Microelisa Stripplate, Standard , Enzyme conjugate, Antibody working solution , Concentrated Wash Solution 20×, concentrated Redissolving solution , Substrate A solution, Substrate B solution, Stop Solution, Derivatization reagent.
【Technique Data】
1. Kit sensitivity: 0.02ppb (ng/ml)
2. Reactive mode: 25℃, 45min～15min
3. Detection Limits:
|
Sample
|
Detection Limits
|
|
Tissue, Liver
|
0.4 ppb
|
|
Honey, Milk
|
0.4 ppb
|
|
Milk powder, Egg powder
|
0.4 ppb
|
|
Fish, Shrimp
|
0.1 ppb
|
4. Cross-reaction rate:
|
Name
|
Cross-reaction rate
|
|
AOZ
|
100%
|
|
AMOZ
|
<0.1%
|
|
AHD
|
<0.1%
|
|
SEM
|
<0.1%
|
5. Recovery rate:
|
Sample
|
Recovery rate
|
|
Tissue, Liver
|
80±25%
|
|
Honey, Milk, Prepared intestine
|
75±15%
|
|
Milk powder, Egg powder
|
85±25%
|
【Storage Conditions】
1.The kit shall be stored at [2-8 ℃]. Expiry date: 12 months
2.The opened Microelisa Stripplate can be stored at [2-8 ℃] and avoid damp. Use for at least 2 months.
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