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- alvetex® 3D Cell Culture Technology
- Product Detail
- Company Profile
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alvetex® presented in a multi-well plate format is ideal for short-term cultures and techniques such as transfection.
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alvetex® mounted in our well insert bathes the 3D culture in medium from above and below enabling long-term culture. Our well insert holder mounted in a deep Petri dish provides a larger volume of medium to further optimize growth of long-term 3D cultures, and enables co-culture studies (2D and 3D; or 3D and 3D).
- Tissue processing, fixation, embedding and sectioning
- Histological staining, in situ hybridisation
- Bright-field microscopy and photographic imaging
- Electron microscopy - both SEM and TEM
- Cryostat sectioning
- Immunocytochemistry
- Fluorescence microscopy, confocal, laser capture
- Isolation of viable cells for passaging
- Flow cytometry and cytospinning
- Extraction of nucleic acid and total protein
- Biochemical assays
alvetex® 3D Cell Culture Technology
AMSBIO offers alvetex® a synthetic scaffold for routine 3D cell culture.
Traditionally, cultured cells normally grow on treated-polystyrene 2D surfaces as in standard cell culture plastic-ware. alvetex® presents cells with the equivalent growth substrate but in a 3D format. These materials are readily adaptable to different types of existing tissue culture plastic-ware (e.g. multi-well plates, well inserts). The culture device is pre-fabricated, sterile, is ready to use off-the-shelf and can be handled in a similar manner as standard 2D plastic-ware.In addition to these products, AMSBIO offer the most advanced and extensive range of 3D tools to the life science community. Want to learn more about it? Have a look at our '3D Cell Culture in Drug Discovery and Disease Modeling' Webinar!
alvetex® Benefits
Consistent Structure
Based on existing cell culture material
Stable and inert
Adaptable to existing cell formats
Compatible with current methods of analysis
alvetex® is a highly porous polystyrene scaffold engineered into a 200µm thick membrane which provides a 3D space into which cells can invade, proliferate and grow. The vertical axis of the third dimension essentially enables additional layers of cells to form complex 3D interactions with adjacent cells simulating the structure of a tissue.
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Product Range
alvetex® is available in a range of formats designed with ease of use and flexibility in mind:
*Inserts not included
- Download alvetex® Format Guide
- Download alvetex® Scientific Articles Review
- Download Hints & Tips for Successful Confocal Microscopy of 3D Cell Cultures
alvetex® advantages
These are distinct advantages over existing 3D culture products which are technically more difficult to use, have a finite shelf life and are expensive.
Importantly, alvetex® can be used for routine 3D cell culture, as an inert plastic, alvetex® is a 3D culture product which can be treated in the same manner as traditional 2D cell culture plastic. The scaffold can be plasma treated without any detrimental effect to its structure. alvetex® is sterilised using gamma radiation. In other words, its manufacture is compatible with standard culture-ware production. Furthermore, the use of polystyrene as a cell growth substrate is well accepted and recognised. These are essential attributes that make alvetex®attractive as a solution for routine 3D cell culture in any research laboratory culturing cells.
alvetex® 3D cell cultures can be studied using a variety of standard molecular and cellular assay techniques.
Assays compatible with alvetex®
Technical Downloads
Go to our alvetex® video presentations
alvetex® Technology
Evidence for the functional superiority of cells cultured on 3D supports compared to 2D monolayer cultures has created much interest in the fabrication of materials that promote the growth and function of cells in a more realistic manner. Cells that make up tissues in the body possess a complex three-dimensional (3D) architecture which is markedly different from the flat monolayer structure of their cultured counterparts commonly grown on the two-dimensional (2D) surface of tissue culture plastic.
alvetex® provides a thin membrane of micro-cellular polymer with a well defined and uniform architecture (images below). alvetex® is made from inert polystyrene using emulsion templating (Barbetta et al. 2005) and its surface chemistry can be varied to influence cell adhesion, proliferation and function (Hayman et al. 2005). By changing the chemical composition of the emulsion and the processing conditions, the porosity of alvetex® can be adjusted to suit alternative applications (Carnachan et al. 2006).
The architecture of the emulsion-templated polystyrene scaffold is revealed below using scanning electron microscopy. The structure of the 200 micron thick membrane is highly porous and consists of a voids linked to one another by pores. The scanning electron microscope image below shows the edge of such a membrane. Note the scale bar for reference.
Traditionally, cultured cells normally grow on treated-polystyrene 2D surfaces as in standard cell culture plastic-ware. alvetex presents cells with the equivalent growth substrate but in a 3D format. These materials are readily adaptable to different types of existing tissue culture plastic-ware (e.g. multi-well plates, well inserts). The culture device is pre-fabricated, sterile, is ready to use off-the-shelf and can be handled in a similar manner as standard 2D plastic-ware.
Tested on a broad and growing selection of different cell types this 3D scaffold demonstratse enhanced functional activity compared to cells grown under identical conditions on 2D culture plastic. Having shown that the differentiation of cultured stem cells is significantly influenced by 3D growth (Hayman et al. 2004).
Related Areas
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AMSBIO’s mission is to be a profitable premier provider of quality life science research reagents and services helping customers develop innovative methods, processes, products and medicines. This is achieved by offering small and medium size manufacturers, academic groups and revenue generating biotechs a unique partnership for the global market and by providing state of the art and cost effective solutions to end users and partners.
Formed in 1987 by Sandy Allan and Alex Sim the company’s first offices were in Madrid, Spain. Over the following three years further offices were opened in Italy, Switzerland and the UK. During the formative years of existence AMS helped launch the successful sales campaigns of Stratagene and Invitrogen providing them with an important foothold from which to develop their global brand expansion.
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