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- 4C5 Anti-DDK Monoclonal Antibody
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4C5 Anti-DDK Monoclonal Antibody
4C5 anti-DDK antibody for the detection of DYKDDDDK epitope (same epitope as Flag®) The DDK-tag is included in the pCMV6-Entry vector that forms the standard backbone of theTrueORF cDNA series. Validated for Western blot, immunoprecipitation, immunofluorescence and flow cytometry Higher sensitivity and specificity compared to other vendors anti-flag antibodies. Purify recombinant proteins with high purities and yields Detect recombinant proteins with low expression levels 4C5 anti-DDK antibody has already been used to: Validate the recombinant protein expression from more than 15,000TrueORF cDNA clones and VERIFY(tm)overexpression lysates Purify more than 5,000 human recombinant proteins from HEK293 cells Purify immunogens under native conditions for the development of TrueMAB antibodies
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Immunoblot analysis of 7 randomly picked VERIFY(tm) myc-DDK tagged overexpression lysates and myc-DDK tagged Western molecular weight marker (MWM1001) using 4C5 anti-DDK antibody at 1:2000 dilution
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| Hela cells transiently transfected with myc-DDK tagged GFAP ORF cDNA clone were immunostained with anti-DDK (1:1000)and stained with Alexa-408 conjugated secondary antibody (1:2000). The nuclei (Blue) were counter-stained with DAPI. |
HEK293T cells transfected with either myc-DDK tagged PROM2 ORF overexpression plasmid (red) or empty vector control plasmid (Blue) were immunostained with anti-DDK antibody and analyzed by flow ctyometry. |
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AMSBIO’s mission is to be a profitable premier provider of quality life science research reagents and services helping customers develop innovative methods, processes, products and medicines. This is achieved by offering small and medium size manufacturers, academic groups and revenue generating biotechs a unique partnership for the global market and by providing state of the art and cost effective solutions to end users and partners.
Formed in 1987 by Sandy Allan and Alex Sim the company’s first offices were in Madrid, Spain. Over the following three years further offices were opened in Italy, Switzerland and the UK. During the formative years of existence AMS helped launch the successful sales campaigns of Stratagene and Invitrogen providing them with an important foothold from which to develop their global brand expansion.
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