The kit is for the quantitative level of CORT in the sample, adopt purified Bird CORT to coat microtiter plate, make
solid-phase antibody, then add CORT to wells, Combine CORT antibody with labeled HRP to form antibody-antigen
-enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color
at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured
at a wavelength of 450 nm. The concentration of CORT in the samples is then determined by comparing the O.D. of the
samples to the standard curve.
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