This ELISA kit uses Sandwich-ELISA as the method. The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of FGF-21 in the sample, this FGF-21 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus FGF-21 concentration. The concentration of FGF-21 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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