FOR RESEARCH USE ONLY. Not for clinical diagnosis use
For the quantitative determination of Human TNF-α concentrations.
Reactivity: Human
Methode Type: Sandwich ELISA Detection
Quantity: 96 tests
Sample type： serum, plasma, Urine,tissue homogenates, cell culture supernates
Detection range : 7 ng/L -400 ng/L
Components:

Product Principle:
The kit is for the quantitative level of TNF-α in the sample, adopt purified Human TNF-α antibody to coat microtiter plate,
make solid-phase antibody, then add TNF-α to wells, Combine TNF-α antibody with labeled HRP to form antibody-antigen
-enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at
HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a
wavelength of 450 nm. The concentration of TNF-α in the samples is then determined by comparing the O.D. of the samples
to the standard curve.

Expiration:
Twelve months [see label on the outer box for the specific date] Storage conditions:
The unopened kit shall be stored at [2-8 ℃] For opened kit can be stored at [2-8 ℃] for up to 1 month. If not be used recently, the standard should be kept in -20 ℃

Attention:
 The kit takes out from the refrigeration should be balanced 15-30 minutes in the room temperature, if the coated
ELISA plates have not been used up after opening, the plate should be stored in sealed bag.
 Washing buffer will Crystallization separation, it can be heated in water to dissolve.
 Pipette sample with pipettors each step, and proofread its accuracy frequently to avoid the experimental error. Pipette
sample within 5 min, if the number of sample is big, recommend using multichannel pipettor.
 If the testing material concentration is excessively high (The sample OD is higher than the first standard well)， please
dilute the sample (n-fold).
 Adhesive Strip only limits the disposable use to avoid cross-contamination.
 The substrate should evade the light to be preserved.
 Please refer to the user instruction strictly, the test result determination must take the microtiter plate reader as a
standard.
 The preparation of samples and all the reagents should refer to infective material process.
 Do not mix reagents with those from other lots.
Washing method:
Manually washing method: shake away the remained liquid in the enzyme plates; place some bibulous papers on the
test-bed, and flap the plates on the upside down strongly. Inject at least 0.35ml after-dilution washing solution into the well,
and marinate 1~2 minutes. Repeat this process according to your requirements.
Automatic washing method: if there is automatic washing machine, it should only be used in the test when you are quite
familiar with its function and performance .